WGS of Siamang: lymphoblastoid - SRA

SRX21899207: WGS of Siamang: lymphoblastoid
1 PACBIO_SMRT (Sequel II) run: 0 spots, 0 bases

Design: PacBio HiFi sequencing at the University of Washington. High-molecular-weight DNA was isolated at PSU from each cell line (5 mln. cells) using the Monarch HMW DNA Extraction Kit for Blood and Cells (New England Biolabs). PacBio HiFi data were collected from each sample as described in (Mao et al. 2023). DNA quantity was assessed at receipt at the University of Washington PacBio Sequencing Services facility and at each subsequent step using the Qubit High Sensitivity DNA kit (ThermoFisher) read on a DS-11 FX instrument (DeNovix) and DNA fragment length distributions evaluated on a FEMTO Pulse capillary electrophoresis instrument (Agilent). Specifically, DNA was sheared using Megaruptor 3 (Diagenode) using settings to target 20kb mode insert length. SMRTbell libraries were generated with the Express Template Prep Kit v2 (PacBio) (all samples) or SMRTbell Prep Kit v3 (PacBio) (Jim_KB3781_GGO) according to manufacturers protocols. Size selection was performed with PippinHT (Sage Science) using a 15- or 17-kbp high-pass protocol. All HiFi libraries were sequenced at the University of Washington on a Sequel II instrument using 30-hour movie times and 2-hour pre-extension. Data sets were generated with P2/C2 chemistry (AG05252_PPY, AG06213_PAB, Jim_KB3781_GGO, PR00251_PPA), P2.2/C2 chemistry (AG05252_PPY, AG06213_PAB, AG18354_PTR, Jambi_SSY, Jim_KB3781_GGO, PR00251_PPA), or P3.2/C2 chemistry (Jim_KB3781_GGO).

Submitted by: University of Washington

Study: Symphalangus syndactylus isolate:Jambi Raw sequence reads

PRJNA976702 • SRP448402 • All experiments • All runs

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This data was generated to assemble the Symphalangus syndactylus cell line Jambi (ToLID: mSymSyn1). The genome was sequenced with multiple technologies.

Library:

Name: m64076_211111_101817_2

Instrument: Sequel II

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Run	# of Spots	# of Bases	Size	Published
SRR26187443				2023-09-27

ID:: 29794387

SRA

Sequence Read Archive

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